I decided that each actor/actress can only make it on the list once. Founded harder to find 50 women, but easier to order them, opposite problem for the men.
Male
1 F. Murray Abraham - Amadeus
2 Marlon Brando - Last Tango in Paris
3 Daniel Day Lewis - There Will Be Blood
4 Bill Murray - Groundhog Day
5 Peter O'Toole - Lawrence of Arabia
6 George C. Scott - Dr. Strangelove
7 Jack Nicholson - The Shining
8 Robert Deniro - Taxi Driver
9 David Thewlis - Naked
10 Paul Newman - Cool Hand Luke
11 Al Pacino - Godfather Part 2
12 Humphrey Bogart - Casablanca
13 Anthony Hopkins - Remains of the Day
14 Peter Falk - A Woman Under the Influence
15 Alain Delon - Le Samourai
16 Ulrich Mühe - The Lives of Others
17 John Turturro - Barton Fink
18 Christoph Waltz - Inglorious Basterds
19 Jean-Pierre Léaud - 400 Blows
20 Erland Josephson - Scenes from a Marriage
21 Dustin Hoffman - Graduate
22 James Stewart - Mr. Smith Goes to Washington
23 Mathieu Almaric - Kings and Queen
24 Klaus Kinski - Aguirre
25 Peter Finch - Network
26 Mickey Rourke - Wrestler
27 Jack Lemmon - Glengary Glen Ross
28 Toshirô Mifune - Seven Samurai
29 Tony Leung - In The Mood For Love
30 Jim Carey - Eternal Sunshine
31 William Macy - Fargo
32 John Goodman - Big Lebowski
33 Javier Bardem - No Country for Old Men
34 Jason Schwartzman - Rushmore
35 Harry Dean Stanton - Paris, Texas
36 Kevin Spacey - American Beauty
37 Marcello Mastroianni - 8 1/2
38 Nicholas Cage - Adaptation
39 Orson Wells - The Third Man
40 John Huston - Chinatown
41 Johnny Depp - Dead Man
42 Steve Coogan - 24 Hour Party People
43 Jeff Daniels - The Squid and the Whale
44 Gene Wilder - Willy Wonka and the Chocolate Factory
45 Michael Keaton - Beetlejuice
46 Jean-Paul Belmondo - Breathless
47 Clint Eastwood - The Good, the Bad and the Ugly
48 Bruno Ganz - Wings of Desire
49 Max von Sydow - The Seventh Seal
50 Edward Norton - 25th Hour
Female
1 Emily Watson - Breaking the Waves
2 Gena Rowlands - A Women Under the Influence
3 Giulietta Masina - La Strada
4 Naomi Watts - Mulholland Dr.
5 Frances McDormand - Fargo
6 Liv Ullmann - Scenes from a Marriage
7 Brenda Blethyn - Secrets & Lies
8 Nicole Kidman - Dogville
9 Ingrid Bergman - Casablanca
10 Elizabeth Taylor - Who's Afraid of Virginia Woolf
11 Gloria Swanson - Sunset Blvd.
12 Rosemarie Dewitt - Rachel Getting Married
13 Julianne Moore - Safe
14 Anamaria Marinca - 4 Month, 3 Weeks, 2 Days
15 Kate Winslet - Eternal Sunshine
16 Juliette Binoche - Blue
17 Anne Baxter - All About Eve
18 Anne Hathaway - Rachel Getting Married
19 Maggie Cheung - In The Mood For Love
20 Bette Davis - All About Eve
21 Anna Karina - A Woman is a Woman
22 Irene Jacob - Double Life of Veronique
23 Faye Wong - Chungking Express
24 Kim Novak - Vertigo
25 Scarlett Johansson - Lost in Translation
26 Emma Thompson - Remains of the Day
27 Julie Delpy - Before Sunset
28 Grace Kelly - Rear Window
29 Barbara Stanwyck - Double Indemnity
30 Imelda Staunton - Vera Drake
31 Cecilia Roth - All About My Mother
32 Anne Bancroft - The Graduate
33 Isabelle Huppert - The Piano Teacher
34 Jeanne Moreau - Jules et Jim
35 Corinne Marchand - Cleo from 5 to 7
36 Carey Mulligan - An Education
37 Shirley MacLaine - Apartment
38 Eva Marie Saint - North by Northwest
39 Helen Mirren - The Cook, the Thief, his Wife and her Lover
40 Holly Hunter - The Piano
41 Ruth Gordon - Harold and Maude
42 Ana López Mercado - Y Tu Mama Tambien
43 Reese Witherspoon - Election
44 Dianne Wiest - Hannah and Her Sisters
45 Louise Fletcher - One Flew Over the Cuckoo's Nest
46 Alexandra Dahlström - Show Me Love
47 Diane Keaton - Annie Hall
48 Jodie Foster - Silence of the Lambs
49 Jean Seberg - Breathless
50 Natalie Portman - Closer